Camv35s polya

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WebApr 7, 2024 · Watermelon (Citrullus lanatus) is an economically important cucurbit crop. Its pulp is rich in antioxidant carotenoids, which confer a variety of flesh colors. ClPsy1 (Phytoene Synthase) is the rate-limiting enzyme for carotenoid synthesis; however, the promoter activity of ClPsy1 is still unknown. In the present study, promoter sequences … Web该【脱水素基因BcDh2及其启动子在培育耐旱植物中的应用的制作方法 1 】是由【421989820】上传分享，文档一共【8】页，该文档可以免费在线阅读，需要了解更多关于【脱水素基因BcDh2及其启动子在培育耐旱植物中的应用的制作方法 1 】的内容，可以使用淘豆网的站内搜索功能，选择自己适合的文档 ...

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WebSep 30, 2005 · October 1, 2005. The Panasonic PV-GS35 is a light and comfortable camera for the casual point and shoot user. Featuring a whopping 30x optical zoom, it can get … http://camera.manualsonline.com/manuals/mfg/polaroid/cta01035s.html city beach thinktime login

pCambia1300 Plant Expression Vector (ab275754) Abcam

WebCaMV35S promoter and terminated by the CaMV35S polyA signal. Reporter genes feature a hexa-Histidine tag at the C-terminus to enable simple purification on immobilised metal affinity chromatography resins. Designed for promoter testing in planta, this vector features a promoterless version of gusA WebCaMV35S promoter, duplicated CreeNd with MpEF1a promoter & 5'UTR 1729bp Stul* attB2 attR1 attR2 CaMV35S terminator polyA signal. CaMV35S promoter, duplicated CreeNd … WebCaMV35S polyA 35S CaMV35S promoter anti-sense sense a b T4 DNA ligase T4 DNA ligase Sac I + Xho I Xba I+ BamH I Xba I+ BamH I . allow inbreeding. A total of 20 random seeds of tra Figure 2. Restriction enzyme analysis of the reconstructs. M, DNA Marker DL-2,000; 1 to 3, pMD18-T-SBE2a/ Apa I+ Xba city beach tennis club wa

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Comparison of a kanamycin versus hygromycin resistance …

WebNptII CaMV35S LacZ alpha CaMV35S CaMV35S polyA uidA Nos polyA LB RB Hind III NcoI AfIII Catalase intron Fig. 1 Schematic representation oftheT-DNAregionofthebinary vector pCambia 2301; LB left T-DNA border, RB right T-DNA border A new high-frequency transformation technique for S. indicum L. 1177. Webby a CaMV35S promoter and terminated by a CaMV35S polyA; (B): same as ‘A’ except that the construct harbored a hygromycin phosphotransferase (hptII) gene. Figure 2. Half … city beach thongsWebSchematic diagram of the T-DNA region of the binary vector pCAMBIA1391; LB: left border, RB: right border, polyA-CaMV: polyA signal, HygR: encoding hygromycin B phosphotransferase, 35S: CaMV35S enhance promoter, MCS: multiple cloning site, GUS: encoding β-glucuronidase, NOS-nopaline synthase terminator. (b). The GUS activity … dicks warehouse aurora oh

"WebOct 14, 2024 · Figure 1.The T-DNA structure of PgCas9/PaU6.The hygromycin phosphate transferase (hpt) gene, which was driven and terminated by the CaMV35S promoter and the CaMV35S polyA, respectively, was used as the selective marker gene. The PgCas9 with a nuclear localization signal (NLS) at each side of 5′ and 3′ ends was driven by the doubled … " - Camv35s polya

Camv35s polya

WebMay 6, 2015 · The CaMV35S polyA module in the plasmid pCAMBIA-1300-221 was selected as the terminator for the basta-resistance cassette, and the entire sequence from the first base immediately after the hygromycin module to the unique SacII restriction site of the plasmid pCAMBIA-1300-221 was amplified with the primer pair CaMV35S-5 and … WebMonday - Friday, EST. 10:00AM - 5:00PM My Account Order Status Wish Lists Sign in Create an Account

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WebNov 4, 2016 · [0203]3-1左侧翼DNA序列检测：利用玉米转化事件的左侧翼基因组序列和外源片段中的35S polyA终止子的序列设计了一对引物(FW-csp2211和RV-csp2344)，建立该转化事件产品的定性PCR鉴定方法。依据ZM8-143转化事件外源DNA片段整合位点LBT-DNA 5’端设计的引物为： Webphotransferase ( hptII) gene in T-DNA region driven by CaMV35S promoter and CaMV35S polyA terminator, which confers resistance to the antibiotic hygromycin as a plant selection marker. Neomycin phosphotransferase (nptII ) gene is located outside the T-DNA region driven by the CaMV35S. Nopaline synthase (NOS)

WebCaMV35S PolyA PMI CaMV35S promoter Ubiquitin pro CryIA(b) gene T-Boder(right) nos BamHI Kpnl Xhol Hindlll pUBB-Man EcoRI XhoI XhoI XhoI A B Figure 1. Diagram of the vector pUBC-Man (A) containing the gene cry1Ac and the vector pUBB-Man (A) containing the gene cry1Ab . Both the two vectors contain the WebOct 15, 2016 · 基因工程第六章植物基因工程.ppt,抗除草剂的转基因植物在大田里，尽管每年花费上百亿美元使用100多种化学除草剂，但杂草的生长仍使农作物减产10%。目前使用的除草剂特异性不强，或多或少会影响农作物的生长。利用转基因技术构建抗除草剂的重组植物可望解决这一问题，其战略包括：抑制农 ...

WebTI’s TPS54335A is a 4.5V to 28V Input, 3A, Synchronous, Step-Down Converter with Eco-mode. Find parameters, ordering and quality information WebJan 3, 2024 · The reporter beta-glucuronidase (GUS) gene was driven by the CaMV35S promoter and terminated by the CaMV35S polyA signal. Successful transformation was detected using polymerase chain reaction (PCR) technology. Detecting a positive result for the T-DNA hptII gene and a negative result for the intercistronic region between Vir B …

WebClone hptII gene into pMD18T to produce pUC18-CaMV35S-hptII-CaMV35S polyA (Table 4) and checked by sequencing (Fig. 5 F). 3. Amplify upstream fragment of Au3446 …

WebMar 8, 2016 · All these genes were driven individually by CaMV35S promoter and CaMV35S polyA terminator (Fig. 1a). The binary vector thus assembled was kindly provided by Dr. P. B. Kirti, University of Hyderabad, Hyderabad, India. The authenticity of the above construct was verified by sequencing of the cloned gene fragments. dick swanson water conditioningWebPlant selection genes in the pCambia vectors are driven by a double-enhancer version of the CaMV35S promoter and terminated by the CaMV35S polyA signal. Reporter genes … city beach tight dressesWebThe present invention relates to nucleic acids and nucleic acid fragments encoding amino acid sequences for flavonoid biosynthetic enzymes in plants, and the use thereof for the m city beach tennis club western australiaWebThis search provides access to all the entity’s information of record with the Secretary of State. For information on ordering certificates and/or copies of documents, refer to the … dicks wapenWebOct 3, 2013 · A total of 29,861 pairs of reads (Supplemental Table S4) mapped to 25 elements in the constructed library, indicating the presence of transgene(s), such as the maize Ubiquitin promoter, CaMV35S ... city beach texasWebNov 25, 2015 · 由此得出，CaMV35S启动PtrpC同样可oxysporum中起作用。然而，本研究同样比较了带有PtrpCCaMV35SpCAMBIA一1300二者转差别很大，前者的转化效率可达到385-460个转化子／10绿木霉分生孢子，而后者转化效率仅为20-50转化子／10绿木霉分生孢子，仅为前者的1／10。 dicks warehouse auroraWebMar 1, 2016 · Development of mASAL expressing transgenic plants. The plant expression cassette comprising cauliflower mosaic virus 35S (CaMV35S) promoter, a 333 bp mASAL coding sequence and a nos terminator was cloned into HindIII/EcoRI site of pCAMBIA1301.The recombinant clone was designated pCAMBIA1301mASAL (Fig. 1) … dickswarehouse.com