Hoechst fluorescent dye
NettetHoechst 33258, DAPI and Vybrant DyeCycle are commonly known DNA fluorescent dyes that are excited by UV and emit in the blue region of the spectrum of visible light. … NettetDescription: IC50 Value: N/A Hoechst stains are part of a family of blue fluorescent dyes used to stain DNA. These Bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33342nd compound made by the company. There are three related Hoechst stains: Hoechst …
Hoechst fluorescent dye
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NettetFluorescent dyes with aromatic amino or guanidine groups, such as propidium iodide (PI), diaminophenylindole (DAPI), acridine orange (AO), and Hoechst dyes, interact with nucleotides to emit fluorescence. PI molecules intercalate inside the DNA double helix. DAPI and Hoechst dye molecules attach at the minor groove of the DNA double helix. NettetNear-Infrared Calcium Dyes: BioTracker NIR Ca 2+ dyes (SCT021, SCT022, SCT023) are far-red fluorescent calcium indicators. These dyes changes fluorescent intensity greatly (1000-fold) when it binds to calcium in living cells. ... lower phototoxicity along with multicolor imaging using Hoechst, Fluorescein, Rhodamine, GFP, YFP and RFP etc.
NettetFeatures of Hoechst 33342 Fluorescent Stain: • Hoechst dye—blue fluorescent stain specific for DNA (i.e., nuclei of eukaryotic cells) • Convenient—provided as an easy-to … Properties of Hoechst Fluorescent Dye As a counterstain in fluorescent imaging, … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes Hoechst dyes are blue-fluorescent nucleic acid stains that have multiple … The Thermo Scientific FITC Antibody Labeling Kit is a multi-component kit … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes Nettet1. okt. 2015 · Figure 2: Live-cell imaging with SiR–Hoechst. ( a) Long-term live-cell microscopy with SiR–Hoechst and other far-red DNA dyes. HeLa cells were imaged for 24 h on a wide-field epifluorescence ...
Nettet18. apr. 2024 · Hoechst dyes are among the most popular fluorophores used to stain DNA in living and fixed cells. Moreover, their high affinity and specificity towards DNA make Hoechst dyes excellent targeting moieties, which can be conjugated to various other molecules in order to tether them to DNA. The recent developments in the fields of … NettetHoechst Stains At-A-Glance. Non-intercalating fluorescent dye that binds to adenine-thymine rich sequences in the minor groove of dsDNA. In solution, Hoechst dyes exhibit minimal fluorescence, but upon binding …
NettetBlue = Hoechst 33342 (fluorescent DNA dye). Flow cytometric analysis of Jurkat cells using Phospho-Histone H3 (Ser10) (D7N8E) XP ® …
NettetThe fluorescent dye 4'-6-Diamidino-2-phenylindole (DAPI) is frequently used in fluorescence microscopy as a chromosome and nuclear stain because of its high specificity for DNA. Normally, DAPI bound to DNA is maximally excited by ultraviolet (UV) light at 358 nm, and emits maximally in the blue rang … ipason windows activationNettetAll of these orange- or red-fluorescent SYTO dyes are available individually as solutions in DMSO (Nucleic Acid Stains—Section 8.1) or as components in the SYTO Orange Fluorescent Stain Sampler Kit or the SYTO Red Fluorescent Stain Sampler Kit , which contain 50 µL each of six different orange-fluorescent or seven different red … open source email spam filterNettetHoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. These dyes are widely used in cell cycle and apoptosis studies as nuclear … ipason s6 tower pc thailandNettetHoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish … ipason wifi driver downloadNettetIf you would like to use fluorescent dyes to create a perfectly labeled cell using a fun interactive tool visit invitrogen.com. Epi-Fluorescence Microscopes Unlike standard fluorescence instruments, the epi … ipason windows product keyNettetFluorescent Dye Proliferation Assays CFSE Labeling. 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is a popular choice for measuring the number of divisions undergone by a cellular population. ... Hoechst 33342 is a DNA staining dye that exhibits low cytotoxicity. ipason wifi dongle driverNettetDAPI is a popular blue fluorescent DNA dye. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement. ... On the other hand, Hoechst dyes are membrane-permeant and more often used for live or fixed cell staining and cell cycle analysis. While Hoechst and DAPI show less cytotoxicity than … ipason wireless usb adapter driver